Kedu ihe kpatara eji polyacrylamide maka protein electrophoresis

N'ihe gbasara sayensị ọgbara ọhụrụ, protein electrophoresis na-eguzo dị ka usoro nkuku maka nyocha na ịkọwa protein.N'etiti usoro a bụ ụghaPolyacrylamide, Ngwakọta dị iche iche nke na-eje ozi dị ka ọkpụkpụ azụ nke gel matrices eji na gel electrophoresis usoro.Ngwongwo pụrụ iche nke Polyacrylamide na-eme ka ọ bụrụ ngwa dị mkpa maka ndị nyocha na ndị sayensị na-achọ ikpughe mgbagwoju anya nke protein na mmekọrịta ha.

Polyacrylamide, nke a na-akpọkarị PAM, bụ polymer synthetic nke sitere na acrylamide monomers.A na-ekwu na ọ nwere ike ịmepụta ogologo agbụ, na-ebute ihe dị ka gel nke nwere ike nabata ụdị mkpụrụ ndụ dị iche iche.Ngwongwo a na-eme polyacrylamide ka ọ bụrụ ezigbo onye ndoro-ndoro maka ịmepụta matrices porous ejiri na protein electrophoresis.

Protein electrophoresis bụ usoro na-ekewa protein na-adabere na ụgwọ na nha ha.Site n'itinye ihe nlele protein na mpaghara eletrik n'ime polyacrylamide gel matrix, ndị na-edozi ahụ na-akwaga gel na ọnụego dị iche iche, na-eme ka ìgwè dị iche iche nke nwere ike nyochaa na ọnụ ọgụgụ.Nkewa a na-enye nghọta bara uru banyere ịdị ọcha protein, mkpebi ịdị arọ nke molekụla, na ọnụnọ nke isoforms.

Ọrụ Polyacrylamide na Protein Electrophoresis

Nhọrọ nke polyacrylamide maka protein electrophoresis gbanyere mkpọrọgwụ na ọdịdị ya nwere ike ịgbanwe.Ndị ọkà mmụta sayensị nwere ike ịhazigharị ntinye nke gel matrix iji nabata protein nke nha dị iche iche.Nleta dị elu na-emepụta matrices siri ike dabara adaba maka idozi protein ndị pere mpe, ebe a na-eji mkpokọta dị ala maka protein buru ibu.Nke a na-agbanwe agbanwe na-eme ka o doo anya na ndị nchọpụta nwere ike ịhazi nnwale ha iji nweta nkewa na nyocha kacha mma.

Polyacrylamide dị ka aFlocculant

Uru Polyacrylamide gbatịrị karịa ọrụ ya na gel electrophoresis.Ọ na-ahụkwa ngwa dị ka flocculant na ụlọ ọrụ dị iche iche, dị ka ọgwụgwọ mmiri na njikwa mmiri mkpofu.Dị ka flocculant, polyacrylamide na-enyere aka n'ịchịkọta ụmụ irighiri ihe ndị kwụsịrị n'ime mmiri mmiri, na-enyere aka iwepụ ha.Njirimara a na-egosipụta ike dị iche iche nke ogige ahụ yana mmetụta dị ukwuu na sayensị na ụlọ ọrụ.

Ọganihu na Electrophoresis dabere na Polyacrylamide

Afọ ndị na-adịbeghị anya ahụla ọganihu na-aga n'ihu na usoro electrophoresis dabeere na polyacrylamide.Native PAGE, SDS-PAGE, na gel electrophoresis akụkụ abụọ bụ naanị atụ ole na ole nke ka ngbanwe nke polyacrylamide siri mee ka mmepe nke ụzọ pụrụ iche maka nyocha ihe owuwu protein, mgbanwe ntụgharị ntụgharị, na mmekọrịta.Usoro ndị a bara uru na nyocha proteomics na mbọ nchọpụta ọgwụ.

N'ihe gbasara nyocha protein, polyacrylamide na-apụta dị ka ezigbo enyi, na-enyere ndị nchọpụta aka ịbanye n'ime ụwa mgbagwoju anya nke protein.Ọrụ ya dị ka ntọala nke gel matrices na usoro electrophoresis enweghị ike ikwubiga ya ókè.Site na usoro ọrịa na-agbasa ruo na-emepụta ọgwụgwọ ọhụrụ, electrophoresis dabeere na polyacrylamide na-aga n'ihu na-akpụzi ọganihu sayensị.Ka teknụzụ na-aga n'ihu, ihe ịtụnanya sịntetị a nwere ike ịmalite, na-emewanyewanye nghọta anyị banyere protein na ọtụtụ ọrụ ha.