Kedu ihe kpatara eji eji Polyacrylamide eme protein electrophoresis

N'ihe gbasara sayensị nke oge a, protein electrophoresis bụ ụzọ dị mkpa isi eji enyocha ma kọwaa protein. Isi ihe dị na usoro a dịPolyacrylamide, ihe mejupụtara ọtụtụ ihe dị iche iche nke na-eje ozi dị ka isi ihe dị mkpa nke matrices gel eji eme ihe na sistemụ electrophoresis gel. Njirimara pụrụ iche nke Polyacrylamide na-eme ka ọ bụrụ ngwa ọrụ dị oke mkpa maka ndị nchọpụta na ndị ọkà mmụta sayensị na-achọ ịchọpụta mgbagwoju anya nke protein na mmekọrịta ha.

Polyacrylamide, nke a na-akpọkarị PAM, bụ polima sịntetik nke e ji acrylamide monomers mee. Ọ na-enye ọtụtụ ihe pụrụ iche site n'ike ya ịmepụta agbụ ogologo, nke na-eme ka ọ bụrụ ihe dị ka gel nke nwere ike ịnabata nha molekul dị iche iche. Ihe onwunwe a na-eme ka polyacrylamide bụrụ ezigbo ihe maka imepụta matrices porous eji eme ihe na electrophoresis protein.

Electrophoresis protein bụ usoro nke na-ekewa protein dabere na chajị na nha ha. Site n'itinye ihe nlele protein n'ime ebe eletriki dị na polyacrylamide gel matrix, protein na-agafe na gel na ọnụego dị iche iche, na-ebute eriri dị iche iche enwere ike inyocha ma tụọ. Nkewa a na-enye nghọta bara uru banyere ịdị ọcha protein, mkpebi ibu molekul, na ọnụnọ nke isoforms.

Ọrụ nke Polyacrylamide na Protein Electrophoresis

Nhọrọ nke polyacrylamide maka protein electrophoresis sitere na ọdịdị ya a na-agbanwe agbanwe. Ndị ọkà mmụta sayensị nwere ike ịgbanwe mkpokọta nke gel matrix iji nabata protein nke nha dị iche iche. Oke mkpokọta na-emepụta matrices siri ike nke dabara adaba maka idozi obere protein, ebe a na-eji obere mkpokọta maka protein buru ibu. Mgbanwe a na-eme ka ndị nchọpụta nwee ike ịhazi nnwale ha iji nweta nkewa na nyocha kacha mma.

Polyacrylamide dị ka iheFlocculant

Uru Polyacrylamide karịrị ọrụ ya na electrophoresis gel. Ọ na-achọtakwa ojiji dị ka flocculant n'ọtụtụ ụlọ ọrụ, dị ka ọgwụgwọ mmiri na njikwa mmiri mkpofu. Dịka flocculant, polyacrylamide na-enyere aka n'ịchịkọta ihe ndị a kwụgidere n'ime mmiri mmiri, na-eme ka mwepụ ha dịkwuo mfe. Àgwà a na-egosipụta ikike dị iche iche nke ihe mejupụtara ya na mmetụta dị ukwuu na sayensị na ụlọ ọrụ.

Ọganihu na Electrophoresis Dabere na Polyacrylamide

Afọ ndị na-adịbeghị anya ahụla ọganihu na-aga n'ihu na usoro electrophoresis dabere na polyacrylamide. Native PAGE, SDS-PAGE, na electrophoresis gel-dimensional abụọ bụ naanị ihe atụ ole na ole nke otu mgbanwe polyacrylamide si enyere aka ịmepụta usoro pụrụ iche maka inyocha nhazi protein, mgbanwe mgbe ntụgharị asụsụ gasịrị, na mmekọrịta. Usoro ndị a bara uru na nyocha proteomics na mbọ nchọpụta ọgwụ.

N'ihe gbasara nyocha protein, polyacrylamide pụtara dị ka enyi siri ike, na-enye ndị nchọpụta ohere inyocha ụwa dị mgbagwoju anya nke protein. Ọrụ ya dị ka ntọala nke gel matrices na sistemụ electrophoresis enweghị ike ikwubiga okwu ókè. Site na ịchọpụta usoro ọrịa ruo n'ịmepụta ọgwụgwọ ọhụrụ, electrophoresis dabere na polyacrylamide na-aga n'ihu na-akpụzi ọganihu sayensị. Ka teknụzụ na-aga n'ihu, ihe ịtụnanya a e ji aka mee ga-agbanwe, na-eme ka nghọta anyị banyere protein na ọtụtụ ọrụ ha ka mma.